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Research

Published findings, in context.

Each finding is shown with the evidence level at which it was observed. A result in an animal model or in cell culture is not a result in humans.

Topic

All topicsTissue damage modelsExtracellular matrixCell signalingCerebral ischemia modelsDermal fibroblasts

Evidence level

All levelsIn vitroAnimalHuman, observationalHuman, controlledReview
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Study type

All typesIn vitro studyAnimal studyObservational (human)Controlled (human)Systematic reviewNarrative reviewCase report
Publication year

3 publications

Compounds filed under Cerebral ischemia models · In vitro

  • In vitroIn vitro study· Rattus norvegicus isolated aorta; cultured vascular endothelial cells

    Isolated rat aorta and cultured endothelial cells: vasomotor tone and Src–caveolin-1–eNOS signaling

    “Modulatory effects of BPC 157 on vasomotor tone and the activation of Src-Caveolin-1-endothelial nitric oxide synthase pathway.”

    Hsieh MJ, Lee CH, Chueh HY et al.Scientific Reports2020PMID 33051481 (opens PubMed in a new tab)DOI 10.1038/s41598-020-74022-y (opens the publisher record in a new tab)

    Filed under BPC-157

    Model
    Ex vivo rat aortic rings with and without endothelium; 3D vascular smooth muscle cell model; cultured endothelial cells
    Exposure
    BPC-157 vs no-peptide preparations (ex vivo and in vitro)
    Comparator
    Preparations without peptide; L-NAME, hemoglobin, Src inhibitor

    In isolated rat aorta, concentration-dependent vasodilation was observed that was attenuated in the absence of endothelium and inhibited by L-NAME or hemoglobin, indicating a nitric oxide-dependent mechanism. In endothelial cells, nitric oxide generation and phosphorylation of Src, caveolin-1 and eNOS increased and were abolished by a Src inhibitor, with less caveolin-1/eNOS association in co-immunoprecipitation assays. No direct relaxant effect on smooth muscle cells was seen in the 3D model.

  • In vitroIn vitro study· Rattus norvegicus (Sprague-Dawley) Achilles-derived fibroblasts

    Rat Achilles-derived fibroblasts: GHR gene and protein expression, proliferation and JAK2 signaling

    Chang CH, Tsai WC, Hsu YH et al.Molecules2014PMID 25415472 (opens PubMed in a new tab)Title on PubMedDOI 10.3390/molecules191119066 (opens the publisher record in a new tab)

    Filed under BPC-157

    Model
    Primary fibroblasts isolated from rat Achilles; cDNA microarray, quantitative PCR, Western blot
    Exposure
    BPC-157, with and without subsequent GHR-ligand stimulation, vs no-peptide culture
    Comparator
    Culture without peptide

    Microarray analysis identified GHR among the most strongly induced genes in Achilles-derived fibroblasts exposed to the peptide; the concentration- and time-dependent increase was confirmed at the mRNA and protein levels. Subsequent stimulation of pre-exposed cells with the GHR ligand was associated with greater proliferation (MTT, PCNA) and JAK2 activation. In vitro observation whose in vivo relevance was not assessed.

  • In vitroIn vitro study· Rattus norvegicus Achilles-derived fibroblasts; Achilles explants

    Rat Achilles explants and Achilles-derived fibroblasts: outgrowth, survival, migration and FAK–paxillin signaling

    Chang CH, Tsai WC, Lin MS et al.Journal of Applied Physiology2011PMID 21030672 (opens PubMed in a new tab)Title on PubMedDOI 10.1152/japplphysiol.00945.2010 (opens the publisher record in a new tab)

    Filed under BPC-157

    Model
    Explant cultures of rat Achilles and primary rat Achilles-derived fibroblasts
    Exposure
    BPC-157 vs no-peptide culture
    Comparator
    Culture without peptide

    In rat Achilles explants, cell outgrowth was faster; fibroblast proliferation (MTT) did not change, but survival under H2O2 stress, transwell migration and cell spreading increased in a concentration-dependent manner, with F-actin formation and higher FAK and paxillin phosphorylation and no change in total protein. The authors propose the FAK–paxillin pathway as a mechanistic hypothesis.

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